Solution-Stock of plasmid dna, You are ready to set up your

Stock of plasmid dna

Reference no: EM131437313

You are ready to set up your reaction. You are provided with a stock of plasmid DNA that is at a concentration of 1mg/ml. You have stock enzyme buffer that is 10X (meaning it is ten-times more concentrated than you need it to be in the actual reaction). You have deionized water (dH₂O) to use, as needed.

How many ml of DNA will you add to your reaction tube?
How many ml of 10X buffer will you add to your reaction tube?
How many ml of ApeKI will you add to your reaction tube?
How many ml of dH₂O will you add to your reaction tube?
At what temperature will you incubate the reaction?

So how would you solve for these?

Reference no: EM131437313

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Stock of plasmid dna

Reference no: EM131437313

Reference no: EM131437313

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