State why was there no increase in the reaction rate

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Why was there no increase in the reaction rate with 8.0 g. of substrate as compared to 4.0 g. of substrate? What would you need to add to see an increase in the reaction rate with 8.0 g. of substrate?

Here is my data

Amount of Substrate

(Lactose) pH 3 pH 5 pH 7 pH 9 pH 11
0.5 g 19 39 72 45 24
1.0g 39 81 145 91 49
2.0g 82 168 300 189 103
4.0g 96 198 350 223 121
8.0 g
96 198 350 223 121

Reference no: EM13177217

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