State what wavelength do measure the change in absorbance

Reference no: EM13207414

Enzyme X has a molecular weight of 48,000. It converts substrate Z into product Y. Z absobs at 340nm, and Y absorbs at 480nm.

1. At what wavelength do you measure the change in absorbance to assay for enzyme X? Does the absorbance increase or decrease over time?

2. If Vmax is 60micromoles/min and you use 400 microliters of a 0.1 mg/mL solution of enzyme, what is the turnover number?

3. Why is the vmax not a constant? Why do we want to analyze Kcat instead of Vmax?

Reference no: EM13207414

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