State what antibiotic put in an agar plate

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Reference no: EM13187062

A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then aneealed with PstI digest of human DNA, ligated and used to transform E.Coli cells. a) what antibiotic-resistance phenotypes will be found on the plate b) what antibiotic woiuld you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid withou an insert? c) which phenotype will indicate the presence of plasmids that contain human DNA fragments?

Reference no: EM13187062

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