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A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then aneealed with PstI digest of human DNA, ligated and used to transform E.Coli cells. a) what antibiotic-resistance phenotypes will be found on the plate b) what antibiotic woiuld you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid withou an insert? c) which phenotype will indicate the presence of plasmids that contain human DNA fragments?
When a sample of Vanillin weighing 2.500g burns in Oxygen, 5.79g of carbon dioxide and 1.18g of water is obtained. What is the empirical formula of Vanillin?
Question enthalpy and entropy values are given and were asked to find boiling point of mercury
calculate the energy in joules and calories. required to heat 5.25g of water, from 55 C to 64.8 C
How many grams of dry NH4Cl need to be added to 1.80 L of a 0.800 solution of ammonia, NH3, to prepare a buffer solution that has a Kb of 8.87? for ammonia is 1.8E-5.
How much does the molar enthalpy change when 147 g. of water cools from 90.0 C to 17.0 C when 20.0 g. of KCL is added to the water? Is this reaction exothermic or endothermic?
Determine ΔG for this reaction When A is full into a cylinder at a pressure of 9.89 atm and the system is permitted to come to equilibrium, the final pressure was 15.94 atm.? Express your answer in units of kJ/mol
Mechanistic rationalization of the effect of the chloro substituent on reactivity (B) Propose an additional experiment to test your mechanistic rationalization IV.
The freezing point depression constant, Kf, for water is 1.86?C/m. Suppose that 5.00 g of mercury(I) nitrate was dissolved in 100.0 g of water
Describe the molecule xenon trioxide, XeO3, using four possible Lewis structures, one each with zero, one, two, or three Xe-O double bonds.
Find the mass of a reactant using Dalton's law of partial pressures, ideal gas law, and stoichiometry - Determine the amount of zinc metal in grams consumed in the reaction.(Vapor pressure of water at 25 o C-23.8mmHg)
How much will the reaction speed increase if the concentration of NO is increased from exactly 0.35 moles/liter to exactly 1.51 moles/liter while the concentration of oxygen
A calorimeter containing 200.0 mL of water was calibrated by carrying out a reaction which released 15.6 kJ of heat. If the temperature rose 3.25 degrees celcius then the heat capacity of the calorimeter inclusing the water is?
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