State the slope and intercept of the best straight line

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Reference no: EM13187655

The data in the table at the right show a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and intercept of the best straight line fit to the calibration curve. (Do not subtract the blank reading when creating your calibration curve.)

RNA (ug) Absorbance at 260nm

0.00 0.299

10.43 0.556

20.86 0.823

31.29 1.187

41.72 1.396

Slope=0.27

intercept=.2872

Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.690.

mass RNA= _____ug

Reference no: EM13187655

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