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The protein insulin contains two polypeptides chains termed the A and B chains. Insulins from different organisms have been isolated and sequenced. Human and chameleon insulins have the same amino acid sequence, with the exception of six amino acids, as shown below. Is the pI of human insulin lower, higher, or the same as that of chameleon insulin?
Amino acid sequence: A8 A9 A10 B1 B2 B27
Human Thr Ser Ile Phe Val Thr
Chameleon Glu Asn Pro Arg Arg Ser
Answer
A) the same
lower
insufficient information
higher
Why?
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