State the recovery of enzyme after the ammonium sulfate step

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Reference no: EM13669983

If an enzyme sample contains 24 mg protein/ml of this sample, 20 micro liters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glycogen at a rate of 1.6 nmol/min. Of this sample, 50mL were fractionated by ammonium sulfate precipitation. The fraction precipitating between30 and 50% saturation was redissolved in a total volume of 10 ml and dialyzed. The solution after dialysis had 12mL and contained30 mg protein/mL. Of the purified fraction, 20 micro liters catalyzed the reaction rate of 5.9 nmol/min under the standard assay conditions. Compute the following:

A. State the recovery of enzyme after the ammonium sulfate step.
B. State the fold purification after the ammonium sulfate step.

Reference no: EM13669983

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