State the protein elutes with the void volume

Assignment Help Chemistry
Reference no: EM13161925

You are developing a purification protocol for a new, uncharacterized protein. When you apply the resin to a gel filtration column, the protein elutes with the void volume. What is the problem? (Note: the column you are using is well packed, and the protein is well within the resolution range of the column.)

Reference no: EM13161925

Questions Cloud

What is the ph of a sodium acetate solution : What is the pH of a sodium acetate solution prepared by adding 0.820 grams of sodium acetate to 100.0 ml of water at 25.0 oC? The Ka at 25.0 oC for acetic acid is 1.8 × 10-5.
Purposes of assessing an economys growth performance : For the purposes of assessing an economy's growth performance, which is the more important statistic, real GDP or nominal GDP? Why?
What is the density of the metal : A piece of metal with mass of 147 g is placed in 50-mL graduated cylinder.The water level rises from 20mL to 41ml. what is the density of the metal?
Cocooning and nesting : Consumers undertaking which of the following would be most likely to be identified as "cocooning" or "nesting"?
State the protein elutes with the void volume : You are developing a purification protocol for a new, uncharacterized protein. When you apply the resin to a gel filtration column, the protein elutes with the void volume. What is the problem?
What are the equilibrium concentrations of no, n2, and o2 : At 2000°C the equilibrium constant for the reaction is Kc = 2.4 x 10^3. , 2 NO(g)-> N2(g) + O2(g) If the initial concentration of NO is 0.100 M, what are the equilibrium concentrations of NO, N2, and O2?
Calculate the number of moles of nitrogen gas produced : A 750-mL sample of nitrogen was collected by displacement of water from a container at 30°C and an atmospheric pressure of 742 Torr. If the vapor pressure of water at 30°C is 31.8 Torr, calculate the number of moles of nitrogen gas produced.
Middle part of the male urethra : The middle part of the male urethra that extends from the prostate to the  penis is called the:
What was the ph of the original hno3 solution : 170mL of an HCl solution with pH of 2.80 is added to 200mL of an HNO3 solution. The resulting pH is 1.40. What was the pH of the original HNO3 solution

Reviews

Write a Review

Chemistry Questions & Answers

  What is the molarity of a solution

What is the molarity of a solution if 4.50 g NaF is dissolved in enough water to make 250.0 mL of solution?

  Carbon and silicon belong to the same group

Carbon and silicon belong to the same group and are known to have similar properties

  Determine the amount of zinc metal in grams

Find the mass of a reactant using Dalton's law of partial pressures, ideal gas law, and stoichiometry - Determine the amount of zinc metal in grams consumed in the reaction.(Vapor pressure of water at 25 o C-23.8mmHg)

  Explain colligative porperties freezing point depression

Colligative Porperties : Freezing Point Depression, Salt lowers the freezing point of water. So will sugar. If one tablespoon of solid (salt or sugar) is dissolved in ¼ cup of water

  Calculate the concentration in ppm of nitrite

A 10mL sample of aquarium water was treated with reagents to form a colour, and after dilution to 100 mL was found to have an absorbance of 0.162. calculate the concentration in ppm of nitrite in the aquarium water.

  How much ligroin would be required

The distribution coefficient, K= conc. in ligroin/conc. in water, between ligroin and water for solute A is 7.5. what weight of A would be removed from a solution of 10g of A in 100 mL of water.

  How many dozens of silver atoms are in jewelry piece

Suppose a piece of silver jewlery contains 5.00 * 10^22 of silver. how many dozens of silver atoms are in the piece of jewlery.

  What is the mass percent of mgco3·3h2o

A mixture of MgCO3 and MgCO3·3H2O has a mass of 3.057 g. After heating to drive off all the water the mass is 2.790 g. What is the mass percent of MgCO3·3H2O in the mixture.

  What is the energy, in joules

A certain radiation has a wavelength of 474 nm. What is the energy, in joules, of (a) one photon

  Calculate the equilibrium constant for the reaction

At 600 degrees C, the equilibrium constant for the reaction 2HgO(s) --> 2Hg (l) +O2 (g) is 2.8. Calculate the equilibrium constant for the reaction 1/2O2(g) + Hg(l) --> HgO (s)

  How much electrical energy (in kw-hr) is consumed

For a current of 360 A at 3.67 V, how much electrical energy (in kw-hr) is consumed in the production of 1 kg of aluminum? (A kW-hr is 3.6 x106 J.)

  What volume of water must be added

A 10.3 mL sample of a HCl solution has a pH of 2.000. What volume of water must be added to change the pH to 4.000?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd