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You have purifiedLDH using affinity chromatography. Using the bradford assay you determined the total protein concentration of the column fraction to be 10 mg/ml in a final volume of 2.4 ml. You diluted some of your column fraction to 1 mg/ml using SDS Page sample loading buffer and loaded 15 ul on to an SDS page gel. You also loaded 10 ul of a 1mg/ml LDH standard in an adjacent lane. The stained sds page gel revealed that your ldh column fraction contained a number of bands besides ldh. Upon scanning you found that the ldh band in your cokumn fraction had an optical density of 42000 pixels. The optical density of the ldh standard band was 58000 pixels.
1. What is the percentage purity of your ldh column fraction?
2. How many total mg of ldh are there in your column fraction?
Using your knowledge of chemistry, describe at least one method that could be used to remove lead(II) acetate from a sample of wine.
Determine the molarity of a saturated aqueous solution of NaI formed as 25degree celsius assuming that the added NaI does not add significantly to the volume of the water?
The active ingredient in Antabuse, a drug used for the treatment of chronic alcoholism, is tetraethylthiuram disulfide, C10H20N2S4 (fw = 296.54). The sulfur in a 0.4329 g
In the qualitative cation analysis procedure, Bi3+ is detected by the appearance of a white precipitate of bismuthyl hydroxide, BiOOH(s): BiOOH(s) --->
The lab was where we had to carry out aldol condensation to prepare benzalacetophenones using C7H5OCl and acetophenone. The result was 4-chlorobenzaldehyde
A Be3+ ion emitts a photon with a frequency of 4.68 × 1016 Hz. If the energy of the initial state of the Be3+ ion was -3.88 × 10(-18) J, calculate the energy of the final state. Express answer in scientific notation.
Water and 8 mol/L potassium nitrate solution are mixed to produce 700mL of a solution with a concentration of 6.mol/L. What volumes of water and potasium nitrate are used.
When 24 mL of a 175 mL stock solution containing 1.6 M BaF2 is diluted to 70 mL, what is the concentration of Ba2+ in the new solution?
What is the concentration of lead ions and sulfide ions in a saturated solution of lead sulfide(PbS) solution at 25 degrees Celsius? (Ksp=3.0x10(to the -10 power)
What is the pH in which 10.0mL of 0.010 mol/L Sr(OH)2 are added to 10.0mL of 0.010mol/L HCl?
How many grams of NaN3 are required to produce 20.0 ft3 of nitrogen gas if the gas has a density of 1.25 g/L?
Calculate the nuclear binding energy of one lithium-6 atom. The measured atomic mass of lithium-6 atom. The measured atomic mass of lithium-6 is 6.015 amu.
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