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You have purifiedLDH using affinity chromatography. Using the bradford assay you determined the total protein concentration of the column fraction to be 10 mg/ml in a final volume of 2.4 ml. You diluted some of your column fraction to 1 mg/ml using SDS Page sample loading buffer and loaded 15 ul on to an SDS page gel. You also loaded 10 ul of a 1mg/ml LDH standard in an adjacent lane. The stained sds page gel revealed that your ldh column fraction contained a number of bands besides ldh. Upon scanning you found that the ldh band in your cokumn fraction had an optical density of 42000 pixels. The optical density of the ldh standard band was 58000 pixels.
1. What is the percentage purity of your ldh column fraction?
2. How many total mg of ldh are there in your column fraction?
What is the average molar mass (in g/mol)? What is the relative standard deviation(RSD) in parts per thousand (ppt)? Report your answer to the correct number of significant figures.
Give the structural formula of the lewis-acid base complex which consists of one mole of phenylmagnesium bromide and two moles of diethyl ether.
Use the stoichimetery of the equation and the dilute titanium 4 chloride solution for the following: How many moles of solid product can be formed?
Calculate tthe freezing point in centigrade units of a soluiton consisting of 685 grams of ethylene glycol dissolved
Assuming that the total pressure of the gases is 1.52 and that their mole ratio is 94 : 4.0 : 1.5 : 0.50, calculate the partial pressure (in atmospheres) of each gas.
The solubility of a gas is 0.34 g/L at STP. What is its solubility at a pressure of 0.80 atm and the same temperature?
A 5.325g sample of methyl benzoate, a compound found in perfumes, was found to contain 3.758g of carbon, 0.316g of hydrogen, and 1.1251g of oxygen.
Calculate the percent relative error for the three solutions if perfect technique is used. Report the two new molarities. (The error in a 10-mL transfer pipet is 0.02, and the error in the 200-mL volumetric flask is 0.1)
A 2.89 g sample of solid calcium carbide (CaC2) reacted with water to produce acetylene gas (C2H2) and aqueous calcium hydroxide.
Calculate the pH of the solution when 20.00 mL of 0.10 M CH3CO2H (Ka= 1.76 x 10^-5) has been titrated
Lead(II) sulfide reacts with hydrogen peroxide to form lead(II) sulfate and water. How many grams of hydrogen peroxide are needed to react completely with 265 g of lead(II) sulfide.
a rock contains .441 g of Pb-206 to every 1.00 g of U-238, assuming the rock did nto cantain any Pb-206 at the timeof its formation, how old is the rock?
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