State the peak absorbance of the first chromatographic peak

Reference no: EM13182244

A mixture of A and B was partially separated on an HPLC column, resultin in overlap of their chromatographic peaks. The absorbance of the effluent was monitored at 300nm (?A = 1800, ?B= 1600) and 350 nm (?A2100 and ?B=3200). The peak absorbance of the first chromatographic peak at 300nm was 0.020 and at 350nm 0.030. What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light path- length = 1.0 cm)

Reference no: EM13182244

Questions Cloud

State benzephenone move on a tlc compared to an alcohol : How would benzephenone move on a TLC compared to an alcohol? and WHY do they move differently.

Describe independent auditors concept of materiality : Briefly describe independent auditors concept of materiality - what are some common measures of materiality with respect to income, sales, and total assets?

State the ph of a citrate buffer composed citric acid : What would be the pH of a citrate buffer composed of 0.35 M citric acid (HCit) and 0.25 M sodium citrate. The Ka of citric acid

Is distance learning equal opportunity for global community : What are your thoughts about the idea that technological and medical advances would bring an end to hunger disease, drudgery and unemployment in the U.S.?

State the peak absorbance of the first chromatographic peak : The peak absorbance of the first chromatographic peak at 300nm was 0.020 and at 350nm 0.030. What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light pat..

Who was virgil : Who was Virgil? What do you suppose prompted him to write the Aeneid? Why did he choose to pattern his epic after Homer's Iliad and Odyssey?

State what is the main component of the first eluting peak : What is the main component of the first eluting peak and what percentage of the total absorbance at 300nm is due to this component?(assuming the light path- length = 1.0 cm)

What strategies did they employ to try gain and hold power : From Marius, through Catiline and Cicero, to Julius Caesar many men sought to seize power in the late Republic. What motivated them? What strategies did they employ to try to gain and hold power? What laws did they pass to ensure their survival, and ..

Prepare general journal entries for the transactions : Prepare general journal entries for the transactions - which had a cost of $5,500 and accumulated depreciation of $5,000, was traded in for a new motor (#23) with a fair market value of $6,500.

User Account

All Pages