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Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the kinetics of this reaction by measuring the conversion of Substance X to Substance Y at various times during purification.
1. Given a pure preparation of these algae, and the required supplies and equipment, devise and outline an empirical procedure for purifying Enzyme X.
2. What is a good indication of purity in your preparation?
The heat of solution of ammonium nitrate is 26.2 kJ/mol. If a 5.368 g sample of NH4NO3 is added to 40.0 mL of water in a calorimeter at 23.5°C, what is the minimum temperature reached by the solution.
If 19.2 g of CO2 is produced in the reaction of C2H2 with O2 to form CO2 and H2O, how many grams of H2O are produced in this reaction.
Calculate the temperature change which will be caused by the following process: 0.640 moles of NH4Cl are dissolved in 1.00 L of water.
Calculate the molar solubility of CaCO3 ( Ksp = 4.5*10-9) neglecting the acid-base character
An enzyme-catalyzed reaction produces a product that has a maximum absorbance at 412nm. The extinction coefficient is 4000M^-1cm^-1, A l0 to-l dilution of the enzyme is made and 20 u.L of the dilution are put into a cuvette with 80 ul of water and..
Identify the species oxidized, the species reduced, the oxidizing agent and the reducing agent in the following electron transfer reaction.
An aqueous solution is six percent by mass methanol,, and has a density = 0.988 g/mL. Determine the molarity of ethanol in the solution.
an adult person radiates heat to the surroundings at about the same as a 100-watt electric incandescent lightbulb. what is the total amount of energy in kcal radiated to the surroundings by an adult in 24 hours?
What is the molarity of a solution prepared by dissolving 13.1 g of glucose (of MW 180 amu) in 217 mL of solution?
What mass of NaCl must be dissolved in 65.0 grams of water to lower the freezing point by 7.50 °C? The freezing point depression constant, Kfp, for water is -1.86 °C/m.
Calculate the pH of a buffer that is 0.158 M HClO and 0.099 M NaClO. The Ka for HClO is 2.9 x 10-8
What mass of NaOH is needed to precipitate the Cd^{2+} ions from 32.0 mL of 0.480 M Cd(NO3)2 solution?
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