State inorganic phosphorous is spectrophotochemically

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Reference no: EM13184378

Inorganic phosphorous is spectrophotochemically measured in blood serum by formation of a reduced heteropoly acid (heteropoly blue) and comparison of its absorbance with a standard treated by the same procedure. If a 1.00 mL blood serum sample gives an absorbance of 0.217, while a 2.00 mL aliquot of a standard containing 91.2 mg KH2PO4 per liter gives an absorbance of 0.285, calculate the milligram percent of P (milligrams per 100.0 mL) in the blood sample. Note: both aliquots are diluted to the same volume prior to absorbance measurements.

Reference no: EM13184378

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