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Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA. THis hydrolytic reaction is catalyzed in E. coli by a pyrophosphatase that has a mass of 120 kd and consists of 6 identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10µmol of pyrophosphate in 15min at 37degrees celsius under std assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.
(a)How many moles of substrate are hydrolyzed persecond per milligram of enzyme when the substrate conc is much greater than KM?
(b)How many moles of active sites are there in 1mg of enzyme?
(c)What's the turnover number of the enzyme?
Which is not evidence that eukaryotes control transcription? a. euchromatin/ heterochromatin b. existence of transcription factors c. lampbrush chromosomes d. occurrence of mutations e. all of these are correct
A patient is scheduled for elective surgery. The patient is currently taking 325 mg of enteric-coated asprin.
This one requires that you think about what limits the respiration rates in states IV, III, and in uncoupled mitochondria. Which of the following situations should produce the fastest rate of respiration? Let the substrate be the same in each case..
In most organisms, oxygen slows down glycolysis, and pyruvate is directed towards the TCA cycle rather than fermentative pathways this is called the Pasteur Effect.
One of the most interesting concepts in animal evolution is the relationship between the phylum Echinoderms and the phylum Chordates. Humans belong to the phylum Chordata.
Describe why asexually reproducing organisms are generally found in environments that do not change very much through time, while sexually reproducing organisms are very successful in environments.
Describe the structure and function of receptors involved in taste and olfaction. Describe how and why each of the following situations would affect someone's perception of flavor.
An individual with a disease demonstrates a set phenotypic presentation, Another individual with the same phenotypic presentation is discovered. When we sequence their genome, we find that there are two distinct DNA sequences involved, both differ..
Think about these two facts, the alpha-subunit of the G-protein trimer has an intrinsic GTPase activity, determine which is important in terminating the action of G-protein coupled systems.
In a flower, what would be the chromosome number of the ovule, embryo sac, and endosperm?
Widespread resistance of malarial parasite to drugs like chloroquine has prompted attempts to develop a malarial vaccine to combat malaria. Explain why is it difficult to develop an effective malaria vaccine?
What are the benefits of genetic engineering versus the potential risks. Critics claimed that the experiment was inconclusive. Which of the criticisms would be addressed by using a swan-necked flask in its place of the unsealed flask.
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