State hydrolytic driving force

Assignment Help Biology

Reference no: EM13178054

Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA. THis hydrolytic reaction is catalyzed in E. coli by a pyrophosphatase that has a mass of 120 kd and consists of 6 identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10µmol of pyrophosphate in 15min at 37degrees celsius under std assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.

(a)How many moles of substrate are hydrolyzed persecond per milligram of enzyme when the substrate conc is much greater than KM?

(b)How many moles of active sites are there in 1mg of enzyme?

(c)What's the turnover number of the enzyme?

Reference no: EM13178054

Questions Cloud

How many bags of cement can be safely lifted on the elevator : An elevator at a construction site has a maximum capacity of 3000 pounds. If the elevator operator weighs 245 pounds and each cement bag weighs 95 pounds, how many bags of cement can be safely lifted on the elevator in one trip?

Assess employee readiness for change-resistance : What did the company do to assess employee readiness for this change? Was there any resistance? Why or why not? How was this managed?

Find a 95% confidence interval for the true percent : A random sample of 700 NAU students in Flagstaff found 287 NAU students who drink coffee daily. Find a 95% confidence interval for the true percent of NAU students in Flagstaff who drink coffee daily. Express your results to the nearest hundredth ..

What was her speed from tortula to cactus : Kiran drove from Tortula to Cactus, a distance of 288 mi. She increased her speed by 8 mi/h for the 392 mi trip from Cactus to Dry Junction. If the total trip took 13 h, what was her speed from Tortula to Cactus?

State hydrolytic driving force : Hydrolytic driving force. The hydrolysis of pyrophosphate to orthophosphate is important in driving forwards biosynthetic reactions such as the synthesis of DNA

Determine a rule for the function f : Julia began driving from Denver to Santa Fe, New Mexico. When she was 120 miles from Denver, she engaged her cruise control to travel at a constant speed. 90 minutes later, she was 223.5 miles from Denver.

Draw the unit circle and plot the point : Draw the unit circle and plot the point P=(3,2). Observe there are TWO lines tangent to the circle passing through the point P. Answer the questions below with 3 decimal places of accuracy.

Calculate the percentage of occupancy for adults : Royal Palm hospital has 500 beds and 55 bassinets. As the HIM manager, you report monthly statical data of hospital operations to administration and department managers. Use the information in table 3-3 to calculate the percentage of occupancy for..

Processes of transportation and deposition : Describe how the processes of transportation, deposition, and lithification influence the character of sediments.

User Account

All Pages