State dtt and laemmli buffer to make the right concentration

Reference no: EM13157994

You have to run SDS-PAGE (a gel electrophoresis method for separating proteins with different MWs). You have to make the protein sample in a buffer called Laemmli buffer (LB) with DTT. The laboratory has a 5M concentration of Laemmli buffer and 1M DTT solution already ready for you. Your final protein sample should be in 1M concentration of LB buffer with 20mM DTT. Your gel sample loading capacity is 50 µL/well. Show how many µL of protein sample you will take and add with the desired volume of DTT and Laemmli buffer to make the right concentration of sample.

Reference no: EM13157994

Questions Cloud

How much would wyandotte have to reduce the price of polyol : How much would Wyandotte have to reduce the price of polyol to achieve a 15 percent increase in the quantity sold?

Explain if specific amount of carbon reacts : If specific amount of carbon reacts with 1.00 g, 1.50 g, and 2.00 g of Hydrogen to form compounds of A, B, and C respectively,

Functionalist and conflict perspectives on power in society : How is power wielded in the social world? Give examples of both the functionalist and conflict perspectives on power in society.

Why might a union be unable to meet its goal : Discuss the conditions that will help the union achieve the objective. Why might a union be unable to meet its goal?

State dtt and laemmli buffer to make the right concentration : Show how many µL of protein sample you will take and add with the desired volume of DTT and Laemmli buffer to make the right concentration of sample.

Identify entrepreneurial projects supported by public funds : Propose at least three methods that governments should employ to reduce their exposure to asymmetric information problems when attempting to identify entrepreneurial projects supported by public funds

What is the final temperature of the silver and water : A piece of silver (25 g) is heated to 100 degrees celsius, then dropped into 42.5 g of water with a temperature of 21.7 celsius. Assume they come to the same temp, what is the final temp of the silver and water? (Molar capacities: Ag 25.351 J/mol*..

Compute theoretical yield for this experiment : calculating theoretical yield for this experiment. It is a dehydration synthesis where one mol of hydrated salt yields 3 mol hydrates and 1 mol of anhydrous salt.

Determine whether it is better to rent or own : Neglect other concerns, like closing costs, capital gains, and tax consequences of owning, and determine whether it is better to rent or own.

User Account

All Pages