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You have to run SDS-PAGE (a gel electrophoresis method for separating proteins with different MWs). You have to make the protein sample in a buffer called Laemmli buffer (LB) with DTT. The laboratory has a 5M concentration of Laemmli buffer and 1M DTT solution already ready for you. Your final protein sample should be in 1M concentration of LB buffer with 20mM DTT. Your gel sample loading capacity is 50 µL/well. Show how many µL of protein sample you will take and add with the desired volume of DTT and Laemmli buffer to make the right concentration of sample.
At a certain temperature and pressure 2.8 L of N2 reacts with 4.2 L of O2. If all the N2 and O2 are consumed, what volume of N2O3, at the same temperature and pressure.
A 2.30 L sample of O2(g) was collected over a liquid at a total pressure of 785 torr and 25°C. When the O2(g) was dried (vapor of that liquid removed), the gas had a volume of 1.86 L at 25°C and 785 torr.
how many grams of chlorine are required to react completely with 5.00grams of sodium to produce sodium chlorde ?
A block of metal has a width of 3.2 cm, a length of 17.1 cm, and height of 3.5 cm. Its mass is 1.2 kg. Calculate the density of the metal.
A saline solution contains 171 mEq/L each of Na+ and Cl-. how many moles of each of Na+ and Cl- are in 1.50 L of the saline solution.
an aerosol spray can with a volume of 270 ml contains 3.2 g of propane gas propellant. what is the pressure in the can at 20 c.
6NO2+2H2O=4(HNO3)+2NO. For this equation, how many grams of nitrogen dioxide are needed to react with 3.70mol of H2O? How many grams of nitrogen monoxide are produced when 2.18mol of H2O react?
What is the first step in the reaction sequence of Aldol reactions under basic conditions? a. Removal of the aldehyde proton b. Removal of an alpha proton to form the enolate
The pressure and colume are simultaneously increased so that at every instant P and v are related by the equation, P = Av, where A is a constant.
A 496-mL sample of a gas at 263 oC is cooled at constant pressure until its volume becomes 241 mL. What is the new temperature in oC?
A solution of ammonia and water contains 3.00×10^25 water molecules and 5.80×10^24 ammonia molecules. How many total hydrogen atoms are in this solution?
The air we breathe is approximetly 20% oxygen. the reaction for the complete combustion of acetylene is 2C2H2 + 5O2 ------> 4CO2+ 2H2o
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