State containing nitrate is reacted with sulfanilimide

Reference no: EM13191315

The nitrite ion (NO2-) is commonly determined by a colormetric procedure using a reaction called the Griess reaction. Im this reaction the sample containing nitrate is reacted with sulfanilimide and N-(1-napthyl)ethylenediamide to form a colored species that absorbs at 550 nm, By using an automated flow aalysis instrument, the followig results were obtained for standard solutions of nitrate and for a sampke containing an unknown amount of nitrite.

Solution Absorbance at 550 nm

Blank 0.002

2.00 ?M 0.065

6.00 ?M 0.205

10.00 ?M 0.388

14.00 ?M 0.474

18.00 ?M 0.598

Unknown 0.402

A) Using any least squares program (linear regression) provide the graph of the best straight line for the data, by plotting glucose concetration (x) vs absorbance (y).*** I already made my graph

B) Provide the equation for the linear fit.

C) Using the equation from (B), determine the concentration of the nitrate ion (mM) in the unknown sample.

D) Using the equation from (B), determie the concentration of the nitrate ion (mM) in the unknown sample.

Reference no: EM13191315

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