State chromatogram to develop for far longer solvent

Reference no: EM13180791

1. Imagine a researcher allowed the paper chromatogram to develop for far longer than necessary in the mobile phase, such that the solvent front ran into the top edge of the paper and the spots continued to move. Would the resulting Retention Factors be overestimated, underestimated or remain unaffected?
Explain your reasoning.

2. Imagine a researcher stopped the development of the paper chromatogram before the solvent front had reached near the top of the paper. Would the resulting Retention Factors be overestimated, underestimated or remain unaffected? Explain your reasoning.

3. Imagine a researcher left the mobile phase sitting uncapped in the fumehood for an extended period of time, such that a large portionof the acetone evaporated. How would this, if at all, change the outcome of the experiment? Would Retention Factors be affected? Would the unknowns still be identifiable? Explain your reasoning.

Reference no: EM13180791

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