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An enzyme-catalyzed reaction produces a product that has a maximum absorbance at 412 nm. The extinction coefficient is 4000 M^-1 cm^-1. A 10-to-1 dilution of the enzyme is made and 20 microliter of the dilution are put into a cuvette with 80 microliter of water and 1.9 mL of reaction cocktail. The absorbance change per minute is 0.05.
(a) How many units are in the cuvette if 1 Units = 1 micromol/min?
(b) How many units are in the cuvette if 1 Units = 1 nanomol/min?
(c) What is the relative activity of the undiluted enzyme (1 Units = 1 micromol/min)?
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Liquid water and nitrogen monoxide gas are produced from the reaction of 22.7 grams of ammonia gas with 53 grams of oxygen gas. 36.0 grams of water were measured at the end of the reaction. What mass of nitrogen monoxide gas was produced from the ..
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