State a reaction mixture containing o-acetyl serine

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Reference no: EM13186065

A reaction mixture containing 20 mM O-acetyl serine, 50 mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine acetylase is incubated in 37C until O-acetyl serine is completely hydrolyzed. What is the pH of the mixture at the end of the reaction?

O-acetly serine, pKa1=1.9, pKa2=8.6

serine, pKa1=2.2, pKa2=9.2

acetic acid pKa=4.76

HEPES, pKa1=3 pKa2=7.6

Reference no: EM13186065

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