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Bradford Assay was performed with samples in triplicate. I got the standard curve and using three abosorbance from the plate reader for the samples and then I extrapolated the protein samples in ug for the triple values and then took a mean of them. Once I got the mean for the samples using the standard curve, I need to find the total protein concentraion of the samples.
How do I find that? I used 50 ul of the sample containing the protein. Let's say that for one of the sample I got the mean of 9 ug using the standard curve, how do I find the total protein concentration for this value (9 ug)? Do I divide 9 ug with 50 ul? Is there a formula I need to use. The total fraction is 500 uL.
A major difficulty in investigating the properties of pancreatic serine proteases is that these enzymes, being proteins, are self digesting. The problem is less severe for solutions of chymotrypsinthan than for solutions of elastase or trypsin.giv..
Work out this chi-square value. How to do a Chi Square trouble for the gentics class.
What does this tell you about the physiological importance of hemoglobin's sigmoidal oxygenbinding curve. Which line represents the bag with the maximum initial concentration of sucrose.
Select an organism which consists of a population having two distinct traits. You can be creative here (red and green beetles, humans with red hair and brown hair, birds with short beaks and long beaks and so on).
Think about a virus with a plus strand RNA genome 8 kb in length, which encodes eight proteins. Determine which of the following is a known mechanism for producing all eight proteins from this genome?
define treatment of phenylketonuria and galactosemia.
He says that the mere presence of DNA is not sufficient to prove the point: his cells may have adopted DNA as a useful molecule quite independently of all other known life forms. What would you do to convince him that he was wrong.
Which of the next statements is untrue of the light reaction in photosynthesis.
For a given reaction, G = -4 kcal/mole and the energy of activation for the uncatalyzed reaction = 5 kcal/mole. You add an enzyme, after which the activation energy decreased to 3 kcal/mole.
Develop an experiment in which you will test the effect of an acidic fluid on enzymatic activity. To complete this assignment, it may be useful for you to review the Scientific Method Tutorial.
Provide many examples of soluble and insoluble dietary fibers. List food sources of these fibers. Contrast the physical characteristics and features of these two types of fiber and their effects on gastrointestinal tract function.
Determine which mode of inheritance produces heterozygotes with phenotypes that differ from homozygote but more closely resembles one homozygous phenotype than other?
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