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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
There are many factors that contribute to the large diversity within bacteria. There are certain factors that allow for increased diversity in bacteria when compared to humans. The following factors contribute to the large diversity found within b..
What would be the predicted numbers of offspring with these four genotypes if the parental generation had been AAbb and aaBB instead of AABB and aabb?
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Mating between black rats of identical genotype produced offspring as follows: 14 cream colored, 47 black, and nineteen albino.
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While limiting the expression of MHC class II to so-called "professional" APCs partially ameliorates the problem of self non self recognition among CD4+ T cells, it remains difficult to understand how it is possible that even these cells' class II..
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q1. in the last few decades remarkable fossils have been discovered indicating that the ancestor of cetaceans whales
In Part One of this case study analysis, students are to use the facts from the case study to determine two different organization theories that are demonstrated. For Part Two, students will compare the 21st century manager to that of the main ch..
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