Source of nutrients except the sugars

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Questions:

Why is it a good idea to read these tubes within a 24 hour period of time?

a) When the bacteria deplete the sugar and start metabolising lactose the media will turn back yellow giving a false positive.

b) When the bacteria deplete all of the protein they will not have any source of nutrients except the sugars and this will give you yellow false positive reaction.

c) The bacteria will ferment all of the available sugar, start deaminating amino acids releasing ammonia and turning the media back to a pink red false negative reaction.

Reference no: EM133411773

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