Sketch the spectrum side chain in the denatured protein

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Reference no: EM13681699

In BPTI the phenylalanine has five aromatic resonances of equal intensity. Though after incubation in 6 M urea the same Phe has three resonances, one of which has half the integrated area of each of the others which have the same area. Define these two spectra as well as the differences between them. As well Sketch the spectrum expected for this side chain in the denatured protein where at H(delta)=7.05, H(epsilon)1= 7.08, H(zeta)1= 7.00ppm.

Reference no: EM13681699

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