Single protein in two different cell lines

Assignment Help Biology
Reference no: EM1382041

Assume you have discovered that a single protein in two different cell lines is significantly overexpressed in one of them. The proteins are identical, yet Northern blot analysis has demonstrated that the mRNAs for the protein in the overexpressing cell line is about 200 base pairs shorter. Subsequent reverse transcriptase PCR has revealed that the truncation occurs in the 3´ untranslated region. How might this lead to overexpression of the protein?

Reference no: EM1382041

Questions Cloud

Macrosociology and microsociology : What is the difference between macrosociology and microsociology? Use an example other than the one cited in this chapter (divorce) to illustrate.
Describe how to improve productivity in non-hospital : describe how to improve productivity in non-hospital organizations e.g. American Heart Association, Lupus Foundation, Alzheimer's Association etc.
Explaining social institutions and economic structures : Describing why the economic social structure called 'middlemen' forms. The literature is replete with examples of how social structures are formed.
Dissecting and analyzing international problem : What methods/procedures/techniques/devices/strategies or approaches would you apply to dissecting and analyzing this international problem.
Single protein in two different cell lines : Assume you have discovered that a single protein in two different cell lines is significantly overexpressed in one of them.
Assume you are about to meet and negotiate : Assume you are about to meet and negotiate with an individual from a low-context culture
Describe the frequency of progeny genotypes : Use chi-square test, test whether these two loci show independent assortment? How would you explain the frequency of the progeny genotypes?
Examining different types of non-parental child care : Critically analyze three different types of non-parental child care. Analyze the influences that non-parental childcare has on psychological, social and cognitive development.
Determine physical address that code byte fetched : Suppose the same code segment base what physical address will code byte be fetched from if instruction pointer contains 539CH?

Reviews

Write a Review

Biology Questions & Answers

  In brief numbered list of events is given for reference

Contained is a description of the signaling cascade that results from EGF stimulation of the Ras-MAPK pathway in cells.

  Functions of muscles

The muscles that originate on the ischium and sacrum are" Adductors of the thigh. Abductors of the thigh. Medial rotators of the thigh.

  What does each of these organelles do in the cell

Activation of T cells requires that antigens be presented from antigen-presenting cells, not simply by exposure to the antigen in solution. What biological rationale may underlie this requirement - consider both from the perspective of how this can a..

  Generate an in vitro system for the formation of copii

Your friend is trying to generate an in vitro system for studying the formation of COPII vesicles. He finds that to get well-organized vesicle budding from artificial membranes, he needs to add the coat proteins, the Sar1 GTP-binding protein.

  Provide an example of convergent and divergent evolution

In what ways are species more "fluid" than even 20th century biologists might have believed? To what extent was Darwin's explanation of species as "convenient constructions" accurate? Are species real.

  Relationship of alcohol use to chd

Determine the suitable measure of the association between alcohol use and CHD in both the smokers and the non-smokers.

  Which commonly applies to hospitality industry

What are the disadvantage and advantage of each and which commonly applies to hospitality industry. How is this caused by the sympathetic nervous division.

  The sequence of the cloned dna

The sequence of the cloned DNA and the amino acids encoded by open reading frames. Stop codons are underlined and labelled 2 or 3 to indicate their reading frame.

  Find the maximum number of genotypes in progeny

In mountain rabbits, the EL-1 gene is located on chromosome three. 7-alleles of this gene have been identified in the population. With respect to EL-1,

  What groove divides cerebrum into two hemispheres

Action potentials are usually generated at axon hillock and propagated down axon away from the cell body. If you experimentally depolarized an axon to the threshold level at a point halfway down the axon, what would happen.

  What proportion of the body weight in phytoplankton

Based on this value, about what proportion of her own body weight in phytoplankton will a 60 kg woman require eating over the course of a year, if this were her only source of energy.

  How can we compare competitive and noncompetitive

how can we Compare competitive and noncompetitive.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd