Single protein in two different cell lines

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Reference no: EM1382041

Assume you have discovered that a single protein in two different cell lines is significantly overexpressed in one of them. The proteins are identical, yet Northern blot analysis has demonstrated that the mRNAs for the protein in the overexpressing cell line is about 200 base pairs shorter. Subsequent reverse transcriptase PCR has revealed that the truncation occurs in the 3´ untranslated region. How might this lead to overexpression of the protein?

Reference no: EM1382041

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