Similarities between transfer and measuring pipettes

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Reference no: EM133253541

Assignment:

Provide answers to the following questions:

• Pipetting technique (exercise 1 -4 )

1. What are the differences and similarities between transfer and measuring pipettes?

2. Describe the appearance of each pipette and its proper use.

3. When do you use a transfer and a measuring pipette?

4. Explain the principle of air displacement and positive displacement pipettes.

5. What are the advantages and disadvantages of using automatic pipettes?

6. Enumerate and explain the parts of an air displacement pipette.

7. Explain the difference in filling and emptying mechanism of automatic pipettes compared serological and Mohr pipettes.

8. What is the importance of verifying pipette calibration?

9. Why is spectrophotometric procedure important in verifying pipette calibration?

10. Why is there a need to frequently perform a pipette calibration verification?

• Pipetting technique (experiment 2)

1. What are the two general types of micropipette? Differentiate the two.

2. State the reason why the thumb finger is not used in controlling the flow of liquid in the pipette.

3. What are the factors affecting pipetting performance?

1. Why is it important not to use chipped or broken tip pipets?

2. What is the main difference between volumetric pipet and Ostwald-Folin pipet? Tabulate the differences.

3. State the differences between air displacement, positive displacement and dispenser pipets.

4. Why do you have to use the forefinger or index finger in controlling the rate of delivery?

5. Identify the sources of error in pipetting.

6. Why should mouth pipetting be avoided in the laboratory?

Reference no: EM133253541

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