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Sequence the dna on both sides of a single known region

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Reference no: EM131126024

PCR is normally used to amplify DNA between known sequences.

What if you wanted to sequence the DNA on both sides of a single known region? Design a protocol that would allow you to amplify the unknown regions of genomic DNA on both sides of a known region.

Be sure to explain each step. Note: a figure describing the method may be very helpful.

Reference no: EM131126024

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