Separating proteins-peptide drugs from fermentation broth

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Reference no: EM132904283

An experiment was designed to identify a better ultrafiltration membrane for separating proteins and peptide drugs from fermentation broth. Two levels of an additive PVP (% wt) and time duration (hours) were investigated to determine the better membrane. The separation values (measured in %) resulting from these experimental runs are as follows:

a. Compute the estimates of the effects and their standard errors for this design.

b. Construct two-factor interaction plots and comment on the interaction of the factors.

c. Use the t ratio to determine the significance of each effect with α = 0.05. Comment on your findings.

d. Compute an approximate 95% CI for each effect. Compare your results with thosein part (a) and comment.

e. From your results in (a) and (b), build a model that relates the separation to the design factors.

f. From your model, determine the optimal settings to maximize the separation.

Reference no: EM132904283

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