Screening experiment for clones that have egfp gene

Assignment Help Biology

Reference no: EM133610674

Question

1. In your PCR screen, what would you expect to see when you run your PCR product on a gel if you had a negative clone (a clone with no insert)?

a. No band on the gel.

b. One band about 1500 bp (1.5 kb).

c. Two bands. LAB SESSION 8 1. What size do you expect your PCR product to be from the screening experiment for clones that have the egfp gene?

2. What size do you expect your PC product to be from the screening experiment for clones that DO NOT have the egfp gene?

3. True or False: The miniprep protocol you'll be performing uses alkaline lysis to purify the plasmid DNA.

4. True or False: While performing a miniprep, you need to vortex your samples well to ensure complete mixing after the addition of Buffer P2.

Reference no: EM133610674

Questions Cloud

Know how the salt concentration : Suppose that we're testing a recipe to make pickles from cucumbers, and we want to know how the salt concentration

Terrestrial animals that dive lose advantages of breathing : Terrestrial animals that dive lose the advantages of breathing air while they are submerged and generally adapt to the extended stays under water.

Animal species is affected by the availability of resources : The size of individuals in an animal species is affected by the availability of resources

Trait is determined by one gene : A particular species of plants can have yellow or green seeds. This trait is determined by one gene.

Screening experiment for clones that have egfp gene : What size do you expect your PCR product to be from the screening experiment for clones that have the egfp gene?

Developing tolerance to the substances : Ken used alcohol and benzodiazepines for over a year, developing a tolerance to the substances.

Split-brain patient sometimes drew interesting pictures : After a comissurotomy to relieve his epilpesy, a split-brain patient sometimes drew interesting pictures after two related words were presented,

Identify key drivers of these observed changes : Identify key drivers of these observed changes and provide supporting evidence from relevant literature.

Ways to limit sodium intake in young children : Ways to limit sodium intake in young children include all of except: Rinsing frozen vegetables before heating Limiting processed snacks Limiting luncheon

User Account

All Pages