Screening experiment for clones that have egfp gene

Reference no: EM133610674

Question

1. In your PCR screen, what would you expect to see when you run your PCR product on a gel if you had a negative clone (a clone with no insert)?

a. No band on the gel.

b. One band about 1500 bp (1.5 kb).

c. Two bands. LAB SESSION 8 1. What size do you expect your PCR product to be from the screening experiment for clones that have the egfp gene?

2. What size do you expect your PC product to be from the screening experiment for clones that DO NOT have the egfp gene?

3. True or False: The miniprep protocol you'll be performing uses alkaline lysis to purify the plasmid DNA.

4. True or False: While performing a miniprep, you need to vortex your samples well to ensure complete mixing after the addition of Buffer P2.

Reference no: EM133610674

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