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Rotonone inhibits Complex I Cyanide inhibits Complex IV Oligomycin inhibits ATP synthase You incubate isolated intact mitochondria in a buffered solution containing NADH, ADP plus Pi. Oxygen is consumed and ATP is made.
1. Upon adding cyanide you examine the effect on oxygen consumption and the production of ATP. After a period of time has passed what will be the effect on: a) oxygen consumption b) ATP synthesis
2. You add the inhibitor rotenone to the respiring mitochondrial preparation. After a period of time has passed, what will be the effect on a) oxygen consumption b) ATP synthesis
3. You add succinate to the rotenone treated mitochondria. After a period of time has passed, what will be the effect on c) oxygen consumption d) ATP synthesis Explain each effect you predict.
Chloroplast thylakoids can be isolated and purified for biochemical experiments. Probes to measure pH are used to measure the interior thylakoid compartment and solution surrounding the thylakoids.
Assume you were given fifty ml of sewage and asked to determine the number of bacteria in the sample. You began by taking five ml and putting it into 45 ml of sterile water.
Hair is principally comprised of the fibrous protein alpha keratin. If warmed in the presence of moisture and 2-mercaptoethanol, hair can be extended by pulling on the ends.
Thirteen-year-old Ryan has begun to challenge many of his parents' values and to express his own set of highly idealistic standards. Compare and contrast the explanations for Ryan's behaviour that would be given by Kohlberg and by Erikson.
Dentinogenesis imperfecta is a tooth disorder involving the production of dentin sialophosphorprotein, a bone-like component of the protective middle layer of teeth.
define the results of the three chi-squared analyses. what do these analyses tell you about the chromosomal arrangements and the genotypes of plants 1 and 2 and the events that occured during gamete production.
A rapid beat of heart over 100 beats per minute is known as ______. A beat slower than 60 is called ______. Lack of appropriate blood to the heart is known as ______. Rapid shuddering of the heart muscle is called ______.
Filling out two Punnett squares for a cross between a male with blood type B and a female with blood type AB. Build two Punnett squares and answer the following questions about them.
Her breath smells like acetone and her blood glucose test out at 650mg/100ml of blood. She is in acidosis. What hormone must be administered to her and why. Where may she find such DNA.
In the telophase stage of mitosis, microtubules gather along the cell's equator. The purpose of these microtubules is to what.
The median cubital vein typicall used for access to the venous system is a superficial vein that communicates between which vessels?
In a particular squash plant, white fruit is dominant to yellow fruit.A white fruited plant is crossed to a yellow fruited plant.Whe the seeds from this cross are planted.
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