Rid an experimental organism of the function of a gene

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Reference no: EM1391364

If you wish to rid an experimental organism of the function of a gene, you can use RNAi. What would you inject into the organism?
a. ssRNA complementary to cDNA b. dsRNA homologous to the gene c.dsRNA including transposase d. co-suppressor with double-stranded (ds) DNA e. ssRNA complementary to mRNA

You have just obtained 100 kb of human genomic sequence.What methods could be used to identify potential genes in this sequence? (CEHCK ALL THAT APPLY)
a.Compare your sequence to an annotated sequence from another species.b. Probe a whole-genomic tiling array with labelled cDNA. c.Perform a yeast two-hybrid experiment using the GAL4/UAS system. d.Use bioinformatic tools and mathematical algorithms to analyze your DNA sequence
Transcomplementation is a name sometimes given for a test to see whether a gene from one organism can "rescue" a loss-of-function mutant of a different organism with a homologous gene. To which of the following processes might this term apply?
a. replacement of a cdc25- with a cdc13+ gene to rescue a cell cycle dysfunction
b. substitution of a drought-resistant gene from potato plants with a drought-resistant gene from a cactus specie c. replacement of a nonfunctional globin gene with a globin pseudogene d.use of Drosophila heat shock gene promoters to regulate heat shock genes in fugu e.introduction of a human WEE1 gene into a yeast culture with wee1- mutants

Reference no: EM1391364

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