Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Question: You are performing a reverse transcription (RT) reactions for high-throughput analysis using a 96-well plate format. Each 96-well plate is 8 horizontal rows of wells by 12 vertical columns of wells. You need to set up the entire 96-well plate will identical RT components except for the particular primers that you will be using. Each of the 8 columns on the plate will get it's own unique primer pair. The best way to accomplish this is to produce a 1 giant mastermix containing all the necessary components minus the primers, and then aliquot the primers as needed into the 8 columns. RT reactions require the following components: buffer, template RNA, dNTPs, RT polymerase, primers, and water. The final volume of each RT reaction is 25 ul.Reagents available:2X RT Buffer50 uM dNTPs537 ug/ul RNA1.0 units/ul RNA polymerasePrimer mix at 100 uMSterile waterBased on your protocol, each RT reaction requires: 1X RT buffer, 5 uM dNTPs, 50 ng/ul RNA, 0.5 units of RT polymerase, and 10 uM each primer.(A)How much of each component will you need for 1 RT reaction? Show your work(B)How much of each component will you need for a mastermix for all 96 reactions? Show your work.
A population of mice can have either black (B) or white (b) fur and long (L) or short (l) tails. Suppose if this is the gamete pool for the parental generation, what is the expected frequency of B/l:b/l zygotes in the next generation,
A new enzyme you are studying has a Km of 7.0mM and a kcat of 126 per second. What will be the initial rate of reaction in units mM/min when 9.3 nanometer enzyme is reacted with 2.7 mM substrate. Report your answer in units if mM/min to nearest 1m..
Develop a cure for cancer - Your cure may consist of a drug or treatment that would be intended for patients who already have cancer
Here are the distances between adjacent pairs of loci. A map distance of fifty = independent assortment. Please map genes giving distances between adjacent pairs of loci.
Determine which water cycle processes are represented in this model and by what components?
Compare and contrast gene expression in prokaryotes vs. eukaryotes from the standpoint of promoter sequences
Say you injected and x amount of protein onto SEC column and it produced certain peaks. Now say you repeated the experiment and use the same amount of protein.
Which solute(s) were able to pass through 20 MWCO membrane? In accordance with your results, which solute had the highest molecular weight?
Most physiological processes in the body can be regulated at three different levels autoregulation within the organ itself, nervous regulation, and hormonal regulation.
Estimate how many photons of light are minimally required to build one glucose molecule in photosynthesis and describe your answer
Give examples of 2-different relationships that fungi have with other organisms. Indicate the type of relationship and explain why it was classified as such.
Assume you are analyzing the function of an unknown drug that you suspect alters metabolism in human cells. You add the drug in different amounts in a colony of cells and find that above a certain concentration,
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd