Restriction enzyme before purification or ligation

Assignment Help Biology
Reference no: EM131422482

In molecular cloning, why both plasmid and PCR product need to be digested by restriction enzyme before purification or ligation?

Reference no: EM131422482

Questions Cloud

Cut down or biodiversity is reduced : Why does it matter if forests are cut down or biodiversity is reduced?
What idea in assign reading the unthinkable and the mundane : What concept/idea in the assigned reading "The Unthinkable...and the Mundane" by Garry Kasparov do you find most intriguing or useful? Why? How might you or others apply it?
Information on health and wellness : After reading Health and Wellness (Johnson, 2011), visit several of the websites listed in that document as providing more information on health and wellness.
Describe the criminal lifestyle : Describe the criminal lifestyle and then discuss why this lifestyle is attractive to certain people-that is, what do they gain from the lifestyle
Restriction enzyme before purification or ligation : In molecular cloning, why both plasmid and PCR product need to be digested by restriction enzyme before purification or ligation?
Discuss the crime of false imprisonment : Discuss the crime of false imprisonment. Next, debate whether or not the suggested change in Question 3 would allow the court to convict the attacker in order to punish him. Provide a rationale to support your response
How can firms ensure that their code of business ethics : How can firms ensure that their code of business ethics is read, understood, believed, remembered, and acted on rather than ignored? What do you feel is the relationship between personal ethics and business ethics?
Provide example of company that has done a total turnaround : Provide an example of a company that has done a total " turnaround". Determine if the turnaround has been effective and improved its profitability.
Were the medical professionals responsible in a legal way : Looking back at this case, were there other parties the State could have tried to hold accountable for her actions? Were the medical professionals responsible in a legal way? What about her family members

Reviews

Write a Review

Biology Questions & Answers

  Significance of buchners experiments

Which of the following is about the significance of Buchner'sexperiments is INCORRECT?

  Diversity in sexually reproducing organisms

What are some ways to increase genetic variability of diversity in sexually reproducing organisms? compare the flow of electrons  through the cyclice and non cyclic  photophosphorylation  provide examples of photosynthesis for both pathways

  Explain the principle of natural selection

Explain the principle of natural selection, Explain how radiometric dating and the fossil record support the theory of evolution

  Function in aninformational capacity

21.  rRNAs __________.      a.  function in aninformational capacity.      b.  are adapted torecognize and bind other molecules

  What is the probability that both children are normal

A woman with keratosis, a skin condition caused by a rare dominant allele, marries a normal man, and they have two children. What is the probability that both children are normal?

  Hypothesis of factor segregation

In the F2 of his cross of red flowered x white flowered Pisum, Mendel obtained 705 plants with red flowers and 224 with white.

  Ductus arteriosus and the ductus venosus

1) The ductus arteriosus and the ductus venosus are two key vessels in the fetal circulation that are absent in the adult circulation. What are their function in the fetus? Why are they necessary?

  Gene expression

Compare and contrast gene expression in prokaryotes vs. eukaryotes from the standpoint of promoter sequences

  Description the role that colchicine plays in the process

Colchicine inhibits the formation of the spindle fibers. Your instructor is interested in creating a polypoid hybrid between cucumbers and cantaloupe. Describe the steps he would have to go through to get this polyploidy. Include in your descripti..

  Discoveries in dna cell biology evolution

Discoveries in DNA, cell biology, evolution, biotechnology have been among the major achievements in biology over the past 200 years with accelerated discoveries and insights over the last 50 years. Consider the progress we have made in these areas o..

  Nucleotide sequences are there for this region of dna

Recall that DNA is made from a sequence of four nucleotides that differ in terms of which nitrogen base A,T,G,C is present in each nucleotide. Suppose you have a region of DNA which is 100 nucleotides long. How many possible nucleotide sequences are ..

  Constriction of the proximal convoluted tubule

How would the following affect the glomerular filtration rate (GFR) and net filtration pressure (NFP)

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd