responsible for the pH effect on kcat

Reference no: EM13526736

Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs. pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 x 105 sec–1 .

What type of functional group might be responsible for the pH effect on kcat? Justify your answerbased on the "generic" pKa values of functional groups in peptides and proteins.

Reference no: EM13526736

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