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Respective functions and modes of action

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1.Assuming DNA replication proceeds at a rate of 750 bp per second, calculate:

a.The time required for the entire E. coli genome to be replicated

b.The minimum number of replication forks per E. coli plasmid required to allow the bacterium to divide in 20 minutes

2.Approximately how many Okazaki fragments are synthesized in the course of replicating an E. coli chromosome? How many in replicating an "average" human chromosome?

3.What is the nucleophile in the reaction catalyzed by DNA polymerase? What is the significance of this in terms of initiating DNA synthesis?

4.Bacterial DNA polymerase I has three activities: 5 ´3 ´exonuclease activity, 3 ´5 ´exonuclease activity, and 5 ´3 ´polymerase activity.a.What are the respective roles for the two exonuclease activities of Pol I?b.What might be a feature of an E. coli strain that lacks the 3 ´5 ´exonuclease activity?

5.How do DNA gyrases and helicases differ in their respective functions and modes of action?

Reference no: EM132633074

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