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1.Assuming DNA replication proceeds at a rate of 750 bp per second, calculate:
a.The time required for the entire E. coli genome to be replicated
b.The minimum number of replication forks per E. coli plasmid required to allow the bacterium to divide in 20 minutes
2.Approximately how many Okazaki fragments are synthesized in the course of replicating an E. coli chromosome? How many in replicating an "average" human chromosome?
3.What is the nucleophile in the reaction catalyzed by DNA polymerase? What is the significance of this in terms of initiating DNA synthesis?
4.Bacterial DNA polymerase I has three activities: 5 ´3 ´exonuclease activity, 3 ´5 ´exonuclease activity, and 5 ´3 ´polymerase activity.a.What are the respective roles for the two exonuclease activities of Pol I?b.What might be a feature of an E. coli strain that lacks the 3 ´5 ´exonuclease activity?
5.How do DNA gyrases and helicases differ in their respective functions and modes of action?
Locate and article in the business press or professional business publications that addresses one of the learning objectives for the week. This article must have been published within the past 12 months. Write a one-paragraph summary of the ar..
Absorption spectra of a mixture of two compounds (A and B) were recorded at 350 nm and 450 nm as 1.1
Many processes are involved in creating the Resting membrane potential.” Describe the ion and concentration FORCES or PROCESSES that are involved in determining the resting membrane potential.
When the aim of DNA cloning is to produce a particular protein in large quantities by expressing the cloned gene in a bacterial cell
Suppose a certain pipet delivered 129 mg of a solution at 20 °C. If the solution had a density of 1.0359 g/mL, what volume of the solution was delivered?
What does this experiment tell you about the underlying mechanism of the last property of the immune system - non responsiveness to "self?"
Bill also started thinking about the damage herbicides can cause if not used properly. In a paragraph or two, describe one to two of the dangers of atrazine.
Does that make the activation energy negative?
A 4.00g sample of an ideal gas occupies 1.24L at 23 degrees Celcius and 1.12bar. What will be its volume at 14 degrees Celcius and 1.50bar?
What are LTPs (long term potentiation)? Where is it located in the brain?
Identify a toxicant that causes reproductive toxicity. the process by which this toxicant causes reproductive toxicity and the concentration of exposure;
What will be the most likely phenotypic and genotypic ratios when a colorblind woman marries a normally visioned man?
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