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You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations
What is a multiplex PCR? What differs in this reaction from a traditional PCR?
How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.
What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?
What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?
Define incomplete 3' addition. How does this appear on an electropherogram?
What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?
Find an action to decrease the rise time of pulses passing through an amplifier.
A current of 2.00 A flows through a resistor that has R = 4.84 ohm. What is the voltage across the resistor
The bulk modulus of aluminum is 7.1 1010 N/m2. If the density of aluminum is 2.7 103 kg/m3, then what is the speed of sound in aluminum
A heat engine operating at maximum efficiency has an efficiency of 25 percent. The temperature of the cold reservoir is 300 K. Evaluate what is the hot reservoir
A 22 rifle bullet, travelling at 350m/s, strikes a soft wood, which it penetrates to a depthof0.130m. The block of wood is clamped in place and doesn't move. How much time is required for the bullet to stop
One ton of coal is burned by a power plant every minute. The energy produced when one kg of coal is burned is 3.2 x 10^7 joules. calculate the efficiency of the power plant
A daring ranch hand sitting on a tree limb wishes to drop vertically onto a horse galloping under the tree. The constant speed of the horse is 12.0 m/s, and the distance from the limb to the level of the saddle is 4.00 m.
Some birthmarks can be removed by exposure to 585 nm laser light. Pulses are strongly absorbed by hemoglobin in the capillaries in the birthmark, What is the power of the laser pulse
The luminance of direct sunlight on Earth is about 105 lm/m2. Determine the luminous intensity of the Sun
find the components of the hiker's total displacement for the trip. Find the magnitude and direction of the displacement from base camp.
A rocket traveling at 0.450c sets out for the star, which is 4.35ly away from earth. According to the stay-at-home earthlings, how long will the journey last
A piece of glass with a flat surface is at the bottom of a tank of water. what is the wavelength of the light in the water
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