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Question: Assume you have isolated a cell population from bone marrow using as newly discovered CD antigen. You believe that this population has hematopoietic potential. When you grow these cells in culture, they have limited proliferation capacity, which you think qualifies them to be an adult type stem cell. Also, in vitro when differentiated, these cells can give rise to enucleated mature erythrocytes. You know that the ultimate proof to classify them as HSC is to show that they home to the bone marrow in vivo. Which experiment do you perform to show that these cells home?
In a 1973-74 situation comedy All in the Family, the character Archie Bunker says, Let me tell you something about beer, You can never buy beer, you can only rent it.
Analyze the DNA of the Phenylalanine hydroxylase gene in patient with phenylketonuria revealed a mutation in protein coding region whose predicted effect would be to replace the amino acid aspartic acid, with histidine.
Write down a paper describing the scientific method and the fundamentals of research. Address each of the following in your paper:
Discuss how do the amplitudes of the finger twitches with different weights compare to each other? Explain why did the amount of work decrease when heavier weights were used?
A group of Ethiopians were assayed for CCR5-del32 deletion polymorphism. 223 were +/+, 64 were +/del32, and 6 were del32/del32.
In metabolically active cells, protons are pumped out of the mitochondrial such that the pH of the mitochondrial matrix is ?8 compared with the neutral pH.
The bases in this "glyceronucleic acid" are symbolized as B, D, H, and U. The monomers are linked during phospodiester bonds and the bases form hydrogen bonds between the strands according to the following rules; for every B, there are twice as many ..
Discuss and explain the role of antidiuretic hormone and aldosterone in the maintenance of blood pressure. Fairly detailed please.
Assume i f you are performing in-situ hybridization to detect mRNA, why do you use the antisense strand? Are you using RNA or DNA as a probe for mRNA?
A geneticist crosses a plant with blue flowers to a plant with white flowers. The offspring include plants with dark blue flowers (1/4), light blue flowers (1/2),
Know terms sterilization, sanitization, disinfect, degermination, and antisepsis. Explain briefly steam and pressure, tyndilization, pasteurization, and boiling water.
Based on the general permeability equation, net flux of solute across a cell membrane, Jnet (gm/sec), is proportional to the permeability coefficient, P,
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