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Think about a strain of E. coli that has a mutation in the gene encoding the IIA protein of the phosphotransferase system. The transport of glucose is not affected by this mutation; the mutant IIA protein can be phosphorylated and the phosphate group is transferred to glucose just as it occurs in the wildtype strain. However, phosphorylation of the mutant IIA protein does not affect its interactions with LacY and adenylate cyclase; i.e. - the phosphorylated form of the mutant IIA protein interacts with LacY permease and adenylate cyclase in a manner identical to the unphosphorylated form of the wildtype IIA protein. How will the lac operon be expressed in this mutant strain of E. coli in the following conditions?a. pplus glucose, minus lactose:b. p pLus glucose, plus lactosec. iminus glucose, minus lactose:d. mmiinus glucose, plus lactose:
Calculate the steady state concentration of radon in the huse assume the ambient concentration is negligible. What is the genus of these bacteria.
Create an experiment in which you will test the effect of an acidic fluid on enzymatic activity. To complete this assignment, it may be useful for you to review the Scientific Method Tutorial.
Throughout much of Europe, there has been a severe decline in mushroom population. On study plots in Holland, data collected since 1912 indicate that the number of mushroom species has dropped from 37 to twelve per plot in recent years.
The measurements to be comparable in both preparations. Illustrate one experiment you could perform to demonstrate such lack of equivalence of the two cells in the zygote. Noticeably state the expected outcome of your experiment in the reply you prov..
In gel filtration chromatography under native cconditions, a protein of interest elutes with a molecular weight between five hundred and 550 kDA.
You wish to fuse a cDNA sequence to the end of two tandem"HA" antibody epitope tags within the MCS-HAHA region of the vector given below.
At what stage of the cell cycle are the chromosomes satisfactorily condensed that they FIRST become visible under the light microscope.
Why does fluoracetate cause citrate to build up in the Citric Acid Cycle.
Tanzanian mudguppies have either green or purple skin. Assume you are interested in the inheritance of skin color in the mudguppie.
The tRNA in the E site will leave and migrate to its aminoacyl-tRNA synthetase d. where the tRNA will be recharged with the correct amino acid.
You require to treat cells with Arsenite such that it has a final concentration of 10uM once it has been added to the cells.
Write down an essay that describes how you would conduct an experiment for your plant hypothesis include an instance of how you would use this method in a real life situation explain each step of the method as it applies to your web experiment and re..
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