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Question 1: Describe DNA sequence variations that are often observed among individuals of the same species. Discuss the possible phenotypic consequences of these DNA sequence variation, give examples, if possible. Discuss the technologies or methods that are often used in detecting each of these variations.
Question 2: Describe the field of epigenetics study. How doepigenetic modifications affect gene expression and phenotype?
Question 3: Briefly describe the principles and applications of 2-dimensional gel electrophoresis, including 2d DIGE, for analysis of proteins.
Question 4: How does real time PCR work to quantify the initial amount of the DNA template? How should the method be modified to quantify the initial amount of RNA template extracted from tissue or cells?
Assume you have identified a mutant E. coli strain that cannot synthesize histidine. To determine the location of the his- mutation on the E. coli chromosome.
Briefly describe the flow of energy through an ecosystem. How does the movement of energy differ from the movement of mineral nutrients?
We have already been introduced to the heat of vaporization of water which equals 2404.2 kJ/liter, i.e. it takes 2404.2 kJ of heat to convert a liter of water into vapor.
Explain the roles of the CAP binding site and the operating site in relationship to the 3 proteins required for growth on lactose. Why aren't these proteins produced all the time? Explain the differences in function when glucose is absent and lact..
Tremendous development in the arid West of the United States over the past few decades, creating demands for water. How should these demands be met? Should there be limits to this type of developments? What kinds of limits, if any?
How do individuals benefit from inclusive fitness?
In corn plants, a dominant allele I inhibits kernel color, while the recessive allele i permits color when homozygous. A second dominant allele P produces purple kernel color, while the homozygous recessive genotype pp causes red kernels.
What muscles are used to bend the trunk when you touch your toes and what is the action of each muscle?
If I was given a chain of amino acids that make up a protein, knowing that some residues have pka values, how would I go about determining the Pka of the protein as a whole. Do I just add the pka values together?
What are the major structural proteins present in the integument? Where are they found, how are they produced
Each protofilament in a microtubule is assembled from subunits of alpha-tubulin and Beta-tubulin subunits. Please describe how the organization of the subunits contributes to the protofilament's polarity and differences between the two ends.
How do technological optimists view human population trends?
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