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Q. You are working in a human genetics laboratory that studies causes and treatments for eye cataracts in newborns. This disease is thought to be caused by a deficiency in an enzyme called galactokinase, but the human gene that encodes this enzyme has not yet been identified. At a talk by a visiting scientist, you learn about a strain of bakers' yeast that contains a mutation called gal1- in its galactokinase gene. Because this gene is needed to metabolize galactose, the mutant strain cannot grow in galactose medium. Knowing that all living things evolved from a common ancestor and that distantly related organisms often have homologous genes that perform similar functions, you wonder if the human galactokinase gene can function in yeast. Because you have an optimistic temperament, you decide to pursue this line of experimentation. You isolate mRNA gene transcripts from human cells, use reverse transcriptase to make complementary DNA (cDNA) copies of the mRNA molecules, and ligate the cDNAs into circular plasmid DNA molecules that can be stably propagated in yeast cells. You then transform the pool of plasmids into gal1- (gal1 minus) yeast cells so that each cell receives a single plasmid. What do you think will happen when you spread the plasmidcontaining cells on Petri plates that contain galactose as a carbon source? How could this approach help you find the human gene encoding galactokinase?
What is the probability of each of the above arrangements occurring? (v) Given that Julie is a carrier, which of the above arrangements must be correct?
Myelin is important for action potential propagation. Multiple sclerosis is an autoimmune disease where the myelin sheath surrounding CNS axons is progressively lost. How will this degradation affect the generation of action potentials?
An alternative to the anaerobic jar is a candle jar, in which a candle is placed in the jar, lit, and the lid closed to enable the flame to use the available oxygen. Typically, in this system, not all of the oxygen is used. Which types of organism..
Mr. X, husband of Mrs. X, has just been diagnosed with benign prostatic hyperplasia (BPH). He has been set down a drug in the same category as prazosin named tamulosin
A drug 2,4-dinitrophenol can get into mitochondria, and carry portons from the intermembrane space, and into the matrix. Determine the effect of DNP on the ATP synthesis in the presence of oxygen?
A herd of beef cattle has a mean production of four hundred units. Individuals with mean production of 500 units are allowed to interbreed, and mean of their offspring is 425 units.
People with a deficiency in the AIRE protein suffer from a broad spectrum autoimmune disease that involves immune attack against a diverse set of body tissues and organs.
At puberty, the adolescent female body changes in both structure and function of several organ systems, primarily under an influence of changing concentrations of estrogen and other steroid hormones. How can one hormone, such as estrogen, mediate so ..
The PCR-derived DNA fragment used an insert in pUC19 was amplified by thenty-five cycles. Suppose that the PCR amplification process and restriction digestion process are 100% efficient.
Under physiological conditions, [glucose] = 5 mM, [g-6-P] = 80 μM, and Pi = 1 mM. Under these conditions, will the reaction go spontaneously from left to right? [Note: the concentration of water is always taken to be 1 M]
What is the least common DNA lesion.
q1. when doing phunnet squares in the question sometimes it doesnt say if the parents are homozygous or hetrozgous so
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