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Q. The chloride concentration in the ACSF used in a lab is 131.5 mM, as the concentration in the internal solution used to fill patch electrodes is 4.4 mM. Suppose that you are making a whole cell current clamp recording from a cell using these solutions. You are recording at room temperature (20oC) and have determined that your cell has a resting potential of -70 mV.
A. What is a chloride reversal potential? Show the work. B. If you rapidly add GABA to the bath, will GABA depolarize, hyperpolarize, or not alter the membrane potential? Describe.
C. Is there a net movement of chloride into or out of the cell while you add GABA to the bath? If so, in which direction?
D. presume you switch your internal solution to one containing 25 mM chloride and obtain a whole cell recording from a new cell that is also resting at -70 mV. What effect will the addition of GABA to the bath have on the membrane potential of this cell? On net direction of chloride movement into or out of cell?
suppose you attempt to isolate different bacterial species from a plate streaked with a mixed population. Would you pick a colony not located on a streak line? A subsurface colony?
You have 0.05 mL of an undiluted culture at a concentration of 3.6 x 106 CFU/ml. You then add 4.95 mL sterile diluent. what is the dilution, and what is the final concentration of the cells?
Suppose that the field of view is at 400x total magnification is 0.26mm. Determine the height of onion cells pictured below from and image observed at 400x.
In his studies of glucose metabolism by yeast, Louis Pasteur discovered that the sudden addition of oxygen (O2) to a previously anaerobic suspension of yeast in grape juice resulted in a dramatic decrease in the rate of glucose consumption.
If populations are sustaining growth at r = 0.088 and the population in 2001 is 3000 individuals, in what year would snow geese be taken off an endangered species list.
The resting potential of a neuron is approximately -60mV. Neurons maintain this electric potential by maintaining a balance of ions inside and outside the membrane. Which of the following is the most significant factor in determining this balance.
How is the gastrointestinal system of a woman affected by pregnancy?
q1. chloramphenicol is the antibiotic that targets prokaryotic 70s ribosomes but not eukaryotic 80s ribosomes. which of
The cuckolded only benefits at the expense of cuckolded perhaps benefits are washed away by loss of paternity in his own nest.
Determine a news report of a current outbreak or other health crisis (within a month) then search for a peer-reviewed scientific article on the same or closely related topic
Discuss what biodiversity means and how it has been affected by natural and man-made pathways.
Diagram (if possible, it would be appreciated, but not necessary) and describe how countercurrent exchange mechanisms work. Use specific examples for: (1) gas exchange; (2) thermoregulation; (3) osmoregulation; and (4) reducing evaporative water l..
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