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Q. Signalling molecules can regulate transcription and as a result control cell differentiation during development. One signaling molecule, sonic hedgehog (shh), has been shown to regulate the dorsoventral patterning of the neural tube.
a) In what tissue is shh produced and what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to produce shh)?
b) Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a).
c) How is the gradient of shh converted into differential transcriptional activation? What I am looking for here is how the investigators determined the stoichiometric relationship between shh gradient and active Gli transcription factors. Describe the experiment that elucidated this relationship. I do not require to have all the details but I want you to describe the experimental approach that was taken, the expression constructs that were generated, how these constructs were delivered to the neural tube and what the results were.
The molecular with of e. coli dna is 2.5 x 10^9. The average molecular weight of a nucleotide pair is 660 and each pair contributes 0.34nm to the length of the DNA molecule. What's the length of the dna molecule?
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