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Q. Once you isolate the cDNA for the receptor involved in mediating heart contraction, you sub-clone receptor cDNA into an expression plasmids and then stably transfect COS cells and choose the receptor-expressing COS-45 cell line. Using your receptor-expressing COS-45 cells, how could you demonstrate experimentally that the selective antagonist binds the receptor specifically? Illustrate the experiment you would perform and also the controls you would contain.
Why are rapidly evolving DNA sequences useful for determining relationships only among taxa that evolved from quite recent common ancestors?
A unit of enzyme activity is usually defined as the amount of enzyme converting 1µmol of substrate per minute under optimum conditions. If the reaction cuvette had a volume of 1mL, calculate how many enzyme units were present in 10µL of enzyme ext..
Describe the processes involved in the transformation of solid materials in rock into soluble nutrients in soil. What biological factors can influence the rate of this transformation?
In D. Melanogaster cherub wings(ch), black body (b) and cinnabar eyes (cn) result from recessive alleles tht are all located on chromosome 2. A homozygous wild-type fly was mated with a cherub, black, and cinnabar fly.
Why was the number of valence electrons excluded from the mystery element data provided?
How would you achieve the same g force with two rotors, one with a radius that is longer than the other? Show your calculation or equation set up.
If a cell were treated with an inhibitor that would cause microtubules to fall apart, which of the following would NOT be affected?
This week's lab activities pertain to taxonomy and classification. For this week's discussion, I would like for you to try to recognize an example in your every day life or work life in which you utilize
First, determine expected frequencies of each individual progeny possibility based on the 44.7 M.U. distance. Do this by showing a punnet square for frequencies.
A tumor is pressing on one cranial nerve. The most significant presenting sign is unequal pupils. Which cranial nerve is probably being compressed?
Explain why people have different skin colors, despite having the same number of melanocytes in their skin.
There are many herbal remedies that state they can detoxify the person by increasing elimination. What dangers are there is using these herbal extracts?
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