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Q. 1.A graduate student is trying to recognize the gene coding for an enzyme found in a bacterial species that degrades trinitrotoluene (TNT). The student is frustrated to find that the organism does not produce the enzyme when grown in nutrient broth, making it difficult to collect the mRNA needed to help identify the gene. What could the student do to potentially enhance the amount of the desired enzyme?
2. You are a student working in a research lab. You isolate a multidrug resistant strain of bacteria from a hospital's intensive care unit. Your multidrug resistant strain can grow in the presence of penicillin, streptomycin, and chloramphenicol. While you mix your multidrug resistant strain with bacteria sensitive to the three antibiotics, you notice that the before sensitive strain is now resistant to penicillin and chloramphenicol, but not streptomycin. How can you describe these results?
3. Why would the series of ribosomal RNA genes remain relatively stable over time?
4. An effective DNA probe can sometimes be developed by knowing the amino acid sequence of the protein encoded by the gene. A student argued that this is too time-consuming as the complete amino acid sequence should be determined in order to create the probe. Does the student have a valid argument? Why or why not with reason?
Organism Profile
Both endocrine and nervous systems are major regulating systems of the body; though, nervous system has been compared to an airmail delivery system and endocrine system to the pony express. In brief explain this comparison.
What is the difference between a root and a rhizoid? What are the "two hallmarks" (p. 574) of alternation of generations? What two stages alternate, and when do mitosis and meiosis happen?
q1. the oldest rocks on earth date from about 4.2 billion years ago. what does this propose about the interval between
Freckles are an autosomal dominant trait. A woman with freckles ( Ff ) marries a man without freckles. What are the chances that their children will have freckles?
Draw the structures of the functional groups ketone and phenyl at high, low and intermediate pH.
Why might replacing an arginine with a histadine result in a defective protein?
Assume that two genes are 55 map units on chromosome II of Drosophila and that a cross is made between a doubly teterozygous female and a homozygous recessive male. What percent recombination would be expected in the offspring of this type of cros..
When doing RAPD PCR, what are the two important changes from the conditions used for standard PCR? (b) When doing RT-PCR (starting with RNA template), what are the two important changes from the conditions used for standard PCR?
Mr. Chow has problem digesting milk products due to a deficiency in the enzyme lactase. His doctor has provided him with a solution of lactase to consume every time he eats a dairy product.
Which is true of the intermediate step of metabolism?
A 26.0mL sample of phosphoric acid (H3PO4) is neutralized by 32.7mL of 2.46M NaOH. A) Balance the equation: NaOH + H3PO4 ? Na3PO4 + H2O B) What is the molarity of the H3PO4 solution?
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