Reference no: EM132680791
1) Explain in one paragraph what SDS-PAGE electrophoresis is.
2) What is the purpose of adding SDS to the protein sample?
3) What is the purpose of adding β-mercaptoethanol to the protein sample?
4) Why are protein samples boiled before they are loaded onto the gel?
5) Could the molecular weight of a protein be estimated if the researcher forgot to add SDS to the protein sample? Explain.
6) The following five proteins, which are listed with their molecular weights and isoelectric points, were separated by SDS-polyacrylamide gel electrophoresis. Give the order of their migration from the top (the point of sample application) to the bottom of the gel.
Molecular
Weight (daltons) pI
a) α-antitrypsin 45,000 5.4
b ) Cytochrome c 13,400 10.6
c) myoglobin 17,000 7.0
d) serum albumin 69,000 4.8
e) transferrin 90,000 5.9