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I use an affinity column to purify a protein, "X" from cell lysate. The specific activity of the protein is very low after this step. So I run the affinity-purified sample through an anion exchanger, and collect the early fraction. This eluate has very high specific activity. My results from a PAGE and Western blot after each step are shown. Also shown are the enzyme kinetics after the affinity purification, and after the anion exchange column. Based on all these data, what is your BEST explanation?
You have 5L of a 0.03 M bicarbonate solution. 1. What is the %mass of the bicarbonate in this solution?2. How much water do you need to add to 59.98g of phosphate to make 3M solution?
Dogs (Canis lupus familiaris) and gray wolves (Canis lupus) can interbreed to produce viable, fertile offspring. These species shared a common ancestor recently.
Retinal detachment from the choroid coat Why
There are at least a dozen known species in the extinct genus of Lystrosaurus . If each species was suited to quitedifferent environments, then this relatively large number ofspecies is likely due to
What concerns would you have about Anton's eating habits? What practical changes could Anton make to make his eating habits more consistent with the Dietary Guidelines for Americans
Assessment: Poster Presentation. For this assessment you are required to present an academic poster which details a proposal of a health promotion intervention to be carried out in the university.
Is there an animal that isan example of Hardy Weinberg Equilibrium? Is there, or has there ever been an animal that isn't evolving, and meets all the rules of the Hardy Weinberg principle?
What would be TRC for a male with the genotype AABbCcDD? For a female. In contrast, each dominant allele in an individual's genotype contributes 12 ridges to the TRC in males and 10 ridges to the TRC in females.
You’ve cloned a 2-kb fragment of DNA from the G38APO8 gene into a bacterial cloning vector and want to construct a restriction map of the insert. You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the e..
Ecosystem
An atom exists in a gas molecule in the atmosphere. It passes by the nodules of a bean plant, where it is converted into ammonia and absorbed into the soil. Bacteria in the soil then convert the ammonia into a form that can be reabsorbed by the bean ..
Affirmative Action Affirmative action is one of the most controversial topics in employment law. How is affirmative action often presented in the media? How similar is that portrait to the one presented in Johnson v. Transportation Agency, Santa Clar..
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