User Account

All Pages

Purification of lactate dehydrogenase

Assignment Help Biology
Reference no: EM131366123

During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml of the A150 buffer?

Reference no: EM131366123

Questions Cloud

Identify the pathophysiological mechanisms of inflammatory : Identify the pathophysiological mechanisms of inflammatory bowel disease and irritable bowel syndrome. Think about similarities and differences between the disorders
Determine the reverse thrust applied by the engine : Determine the reverse thrust applied by the engine to the airplane. Assume the inlet and exit pressures are atmospheric and that the mass flowrate of fuel is negligible compared to the air flowrate through the engine.
Calculate the client target heart rate : Calculate the client's target heart rate using the Karvonen formula.
Read the article competition in america : Read the article Competition in America - Write an essay the directions - In the body of the essay support your support your agreement/disagreement with specific examples from your experience or reading and/or with analysis of the essay's argument
Purification of lactate dehydrogenase : During the Purification of Lactate Dehydrogenase (LDH) experiment, you will need 50ml of buffer A150. Buffer A150 is 30mM Tris (pH 8.8) and 150mM NaCl. Given 1L of 1.5M Tris and 500ml of 5M NaCl, how much of each stock do you need to make 500ml ..
Soil taxonomy is a generic system of classification : 1. What does it mean to say that the Soil Taxonomy is a generic system of classification? 2. Describe and explain the soil horizon development of Entisols. 3. In which kind of climate are Gelisols typically found?
Determine the force produced by the thruster : Assume that the inlet and outlet pressures are zero and that the momentum of the water entering the thruster is negligible.
Difference between soil porosity and permeability : 1. What is the difference between soil porosity and permeability? 2. What is cation exchange capacity and how does it relate to soil fertility?
What is the technology how does it work : What is the technology? How does it work? (This should be only a brief description due to the limited number of pages allowed in this report) How did society function prior to the wide-spread use of the technology or its first use or introduction o..

Reviews

Write a Review

Biology Questions & Answers

  Wha primary difference between passive and active transport

Imagine that you are a pediatrician and one of your patients is a newborn who may have a lysosomal storage disease. You remove some cells from the patient and examine them under the microscope. What would you expect to see? Design a series of test..

  What is the most accepted hypothesis on the origin of life

What is the most accepted hypothesis on the origin of life on earth? How does it compare to the other main hypotheses?

  What is meant by the term true-breeding plants

What is Mendel's second principle? Include in your answer the type of cross that led Mendelto this explanation.

  Homeostaticchange

Which of the following would be a homeostaticchange?

  What are the primary weaknesses of dna microarrays

what are the source of biases in the four core techniques (quantitative realtime PCR, suppressive/subtractive hybridization (SSH), and expression screening this week) described in the lecture material?

  What are telomeres and what is the function

What evidence was utilized to determine which bases pair with each other? What are Telomeres and what is the function? Why are ends of the telomeres lost during DNA replication?

  Which culture had a shorter generation time

Flask A includes yeast cells in glucose-minimal salts broth incubated at 30C with aeration. Flask B contains yeast cells in a glucose-minimal salt broth incubated at 30C in an anaerobic jar.

  Binding ribosomes and synthesizing leader peptides

Create a model that would permit the attenuation region to no longer be involved in binding ribosomes and synthesizing leader peptides after the attenuation decision is made.

  Genetic variability affect the evolution of species

How does this increase in genetic variability affect the evolution of species?

  Do you think chronotype helps you be more or less productive

Do you think your chronotype helps you be more or less productive? Do you think there are any ill effects on your health or social life due to your sleep patterns?

  Explain what is meant by indicator organisms when testing a

explain what is meant by indicator organisms when testing a water sample. what are the media used to determine the

  Which situations violate hardy-weinberg equilibrium

What tends to happen to genetic diversity after a bottle neck or founder event? Is it predictable?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

Submit Assignment

Academics

Major Subjects

Majors

Get In Touch

whatsapp: +1-415-670-9521

Phone: +1-415-670-9521

Email: [email protected]

TERMS & POLICIES

HELP & SUPPORT

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd