Protein product of a humangene in a bacterial host

Reference no: EM13533558

You are interested in overproducing the protein product of a humangene (that has one intron in it) in a bacterial host (whereno splicing of the intron from the mRNA canoccur). To get a copy of the gene without the intron, you try to docDNA cloning starting with poly(A)⁺ mRNA. After severalfailed attempts to find the cDNA clone, you come to the conclusionthat the mRNA is most likely very unstable. Suggest an alternateapproach to producing an intron-free gene, ready for cloning in anexpression vecto

Reference no: EM13533558

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