Protein concentration of an unknown sample

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You obtained the following raw data when setting up a Bradford standard curve:

BSA (mg/ml)Absorbancy 595nm00.22510.31020.42030.51040.61050.72060.81070.91580.95090.980100.990

After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.69 was obtained.

Set up a standard curve, excluding outliers and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).

Reference no: EM132440091

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