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Process of mutating dna with pcr

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In the process of mutating DNA with PCR, the synthetic DNA segment containing the mutation must be inserted back into the recombinant vector. Which enzyme binds the vector and synthetic DNA?

You want to clone a large DNA sequence that is 176 kbp and realize this is too big to insert into a plasmid. What other vector could be used?

Reference no: EM132439707

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