Reference no: EM132412759

Q1) You obtain the sequence of a gene containing 10 exons, 9 introns, and a 3'UTR containing a Poly A sequence. The fifth intron also contains a polyadenylation site. 

To test whether both polyadenylation sites are used, you isolate mRNA and find a longer transcript from muscle tissue and a shorter transcript from all other tissues. Speculate a mechanism in production of these different transcripts.

Q2) RNA editing is a common process in the mitochondria of trypanosomes and plants as well as in chloroplasts, and in rare cases it occurs in higher eukaryotes. What is RNA editing, and what benefit does it demonstrate in the documented example of ApoB in humans?

Q3) During Sxl splicing, how does Tra protein regulate the production of Dsx protein? Describe its interaction with Rbp1 and Tra2, and how they define splicing between exons 3 and 4, rather than 3 and 5.